Analyze your qPCR data in seconds

Relative and absolute quantification with primer efficiency correction. Multi-reference normalization with geNorm M and NormFinder ρ. Levene's variance check with Games-Howell post-hoc for unequal-variance groups.

A 5-parameter logistic fit runs on every well's raw fluorescence. Fit Cq (SDM) — the second-derivative maximum, independent of baseline or threshold position — sits beside the reported Cq for comparison. Per-well Curve Shape QC flags atypical kinetics that threshold methods miss.

Built to the 2025 MIQE 2.0 standard: prediction-interval LOD/LOQ, efficiency-corrected quantities, reference gene stability, NTC validation, and an automated compliance checklist.

Automated experiment interpretation that tells you what your data means — not just what it looks like. Expression overview, quality flags, and suggested follow-up experiments.

Bar charts, heatmaps with hierarchical clustering, PCA, volcano plots, correlation scatter plots, and melt curves — all exportable at 300+ DPI as JPEG, TIFF, or PDF.

Plate heatmaps, replicate SD and CV% flagging, MAD outlier detection, NTC monitoring, and customizable Cq/SD thresholds — all computed instantly on upload.

Multi-page US Letter reports with title page, all figures, MIQE compliance, and Guru Insights. Choose which sections to include. Ready for lab notebooks or publications.

Primer efficiency, LOD/LOQ, and absolute quantification from a dilution series. Per-point diagnostics — residuals, leverage, Cook's distance — let you spot influential points and refit interactively.

Auto-detects 7+ instrument formats. Merge plates from any combination of runs and instruments — inter-run calibrator detection assesses plate-to-plate drift automatically.